University of Southern California

Services/Order Form

To request genome editing or iPSC derivation services, USC laboratories are invited to download and complete the Chang CORE Request Form, and email it to Genome Editing Manager Chang Tong at changton@usc.edu.

Note: Below pricing is for USC laboratories. To obtain pricing for non-USC laboratories or to discuss other services or products, please contact Genome Editing Manager Chang Tong at changton@usc.edu or 323-442-7834.

Genome editing | Human iPSC derivation | Cells and media

Genome editing for human iPSC and ESC

CRISPR/Cas9 technology genome editing procedure Price Time
Step 1: Construction and testing of CRISPR/Cas9 vectors Vector construction: $487 for all-in-one plasmid; $447 for co-transfection of RNA of Cas9 and gRNA. Validation: $405 for T7 endonuclease assay; $495 for fluorescent protein reporter plasmid assay. 10 days
Step 2a: CRISPR/Cas9-mediated gene knock-out $14,428 3 months
Step 2b: CRISPR/Cas9-mediated gene knock-in $15,664 4 months
Step 2c: CRISPR/Cas9-mediated point mutation $14,613 4 months
Step 3: Southern Blot confirmation $1,200 21 days

Human iPSC derivation

Service procedure Price Time
Step 1: Derive iPS line using episomal vector technology. $3,851 per cell line 30 days
Step 2: Characterize iPS cells/confirm undifferentiated phenotype (ScoreCard). $660 2–4 weeks

Cells and media

Product Price
hESCs 35 mm dish: $93; Frozen vial: $608
MEFs $36 per million
mTeSR1, TeSR-E8 $230