With a generous gift from the Chang Family, the Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC founded the Chang Stem Cell Engineering Facility in June 2015. The state-of-the-art facility helps researchers at USC and beyond:
- create tools to study basic biological processes, such as the development of the heart, kidney and liver;
- explore mechanisms underlying various devastating diseases, such as amyotrophic lateral sclerosis, Alzheimer’s disease, autism, pulmonary fibrosis and liver failure; and
- generate tools to treat genetic disorders, such as sickle cell disease, thalassemia and other immune deficiencies.
To assist in these endeavors, the facility genetically modifies embryonic stem (ES) cells and induced pluripotent stem (iPS) cell lines. Genetically modified ES and iPS cell lines derived from disease-specific animal models and patients provide an opportunity to investigate the mechanisms of disease and to further develop new treatments for clinical therapy.
The facility operates on a fee-for-service basis and provides customized ES and/or iPS genome editing to all investigators at USC and beyond.
The state-of-the-art facility is equipped with: one molecular biology room for vector construction, two tissue culture rooms for pluripotent stem cell derivation and expansion, a hybridization oven, an Amaxa nucleofector, a water-jacketed CO2 incubator, a low-oxygen chamber and a BSL-2 tissue culture hood.