To request genome editing or iPSC derivation services, USC laboratories are invited to download and complete the Chang CORE Request Form, and email it to Genome Editing Manager Chang Tong at email@example.com.
Note: Below pricing is for USC laboratories. To obtain pricing for non-USC laboratories or to discuss other services or products, please contact Genome Editing Manager Chang Tong at firstname.lastname@example.org or 323-442-7834.
Genome editing for human iPSC and ESC
|CRISPR/Cas9 technology genome editing procedure||Price||Time|
|Step 1: Construction and testing of CRISPR/Cas9 vectors||Vector construction: $487 for all-in-one plasmid; $447 for co-transfection of RNA of Cas9 and gRNA. Validation: $405 for T7 endonuclease assay; $495 for fluorescent protein reporter plasmid assay.||10 days|
|Step 2a: CRISPR/Cas9-mediated gene knock-out||$14,428||3 months|
|Step 2b: CRISPR/Cas9-mediated gene knock-in||$15,664||4 months|
|Step 2c: CRISPR/Cas9-mediated point mutation||$14,613||4 months|
|Step 3: Southern Blot confirmation||$1,200||21 days|
Human iPSC derivation
|Step 1: Derive iPS line using episomal vector technology.||$3,851 per cell line||30 days|
|Step 2: Characterize iPS cells/confirm undifferentiated phenotype (ScoreCard).||$660||2–4 weeks|
Cells and media
|hESCs||35 mm dish: $93; Frozen vial: $608|
|MEFs||$36 per million|