University of Southern California

Step 2: Reprogram fibroblast using episomal vector technology.

This step takes about 60 days for human fibroblast cells.

1. 3–5 million fibroblast cells are needed.

2. Episomal vectors carrying reprogramming factors are used for transfection.

3. Seed the transfected cells onto two 10 cm dishes.

4. Pick up 2–5 colonies for expansion.

5. Mycoplasma testing and freezing down